Large-scale cultivation of Leishmania infantum promastigotes in stirred bioreactor
نویسندگان
چکیده
منابع مشابه
Evaluating the Coconut Water as a Replacement for the Fetal Calf Serum in Cultivation of Promastigotes of Leishmania Infantum
Backgrounds and Aims: The protozoan parasites of the genus Leishmania can be stated as the causative agents of the various clinical diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, which is the most expensive ingredient in these media. The coconut water is packed with nutrients that yield an array of health benefits. Materials and Methods: In the present st...
متن کاملCanine experimental infection: intradermal inoculation of Leishmania infantum promastigotes.
Five mixed breed dogs were inoculated intradermally (ID) with cultured virulent stationary phase promastigotes of Leishmania infantum Nicole, 1908 stocks recently isolated. Parasite transformations in the skin of ID infected dogs were monitored from the moment of inoculation and for 48 h, by skin biopsies. Anti-Leishmania antibody levels were measured by indirect immunofluorescence assay, count...
متن کاملImpact of Continuous Axenic Cultivation in Leishmania infantum Virulence
Experimental infections with visceral Leishmania spp. are frequently performed referring to stationary parasite cultures that are comprised of a mixture of metacyclic and non-metacyclic parasites often with little regard to time of culture and metacyclic purification. This may lead to misleading or irreproducible experimental data. It is known that the maintenance of Leishmania spp. in vitro re...
متن کاملAnti-Leishmanial Activity of Pelargonium roseum Essential Oil on Growth of Leishmania infantum Promastigotes
ABSTRACT Background and Objectives: Due to adverse effects of pentavalent antimonial treatment of visceral leishmaniasis using herbal medicines has received a lot of attention. In this study, we evaluated inhibitory activity of Pelargonium roseum essential oil on growth of Leishmania infantum promastigotes, and compared it with that ...
متن کاملevaluating the coconut water as a replacement for the fetal calf serum in cultivation of promastigotes of leishmania infantum
backgrounds and aims: the protozoan parasites of the genus leishmania can be stated as the causative agents of the various clinical diseases. fetal calf serum (fcs) is the major part of the leishmania culture media, which is the most expensive ingredient in these media. the coconut water is packed with nutrients that yield an array of health benefits. materials and methods: in the present study...
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ژورنال
عنوان ژورنال: Journal of Vector Borne Diseases
سال: 2019
ISSN: 0972-9062
DOI: 10.4103/0972-9062.302038